The Faculty

Focus

Weinstein Lab develops new modalities of biological measurement, drawing from tools in molecular biology, physics, and applied mathematics

Research Interests

Joshua Weinstein is a biophysicist and molecular technologist. He works on developing DNA-based technologies for high-throughput encoding and decoding of biological information with a view toward applications in biology and medicine. He has previously worked on applying massively parallel DNA sequencing to the study of immune receptor repertoires. Recently, he invented DNA microscopy, an imaging modality that uses DNA rather than light as an imaging medium in order to generate detailed pictures of how genetic diversity distributes spatially across biological specimens.

Selected Publications

  1. Weinstein JA, Regev A, Zhang F. “DNA microscopy: Optics-free spatio-genetic imaging by a stand-alone chemical reaction.” Cell. 2019 Jun 27. (paper, pre-print)
  2. Vollmers C, Sit RV, Weinstein JA, Dekker CL, Quake SR. “Genetic measurement of memory B-cell recall using antibody repertoire sequencing.” Proc Natl Acad Sci USA. 2013 Jul 29; doi:10.1073/pnas.1312146110. (paper)
  3. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. “DNA targeting specificity of RNA-guided Cas9 nucleases.” Nat Biotech. 2013 Jul 21; doi:10.1038/nbt.2647. (paper)
  4. Weinstein JA, Zeng X, Chien YC, Quake SR. “Correlation of gene expression and genome mutation in single B-cells.” PLoS ONE. 2013 Jun 28; 8(6): e67624. (paper)
  5. Jiang N*, He J*, Weinstein JA*, Penland L, Sasaki S, He XS, Dekker CL, Zheng NY, Huang M, Sullivan M, Wilson PC, Greenberg HB, Davis MM, Fisher DS, Quake SR. “Lineage structure of the human antibody repertoire in response to influenza vaccination.” Sci Transl Med. 2013 Feb 6;5(171):171ra19. (paper)
  6. Jiang N*, Weinstein JA*, Penland L, White RA 3rd, Fisher DS, Quake SR. “Determinism and stochasticity during maturation of the zebrafish antibody repertoire.” Proc Natl Acad Sci USA. 2011 Mar 29;108(13):5348-53. (paper)
  7. Weinstein JA*, Jiang N*, White RA 3rd, Fisher DS, Quake SR. “High-throughput sequencing of the zebrafish antibody repertoire.” Science. 2009 May 8;324(5928):807-10. (paper)