RNA biophysics, cell biology
University of Science and Technology China, B.A., 2005
Columbia University, Ph.D., 2010
Columbia University, Postdoctoral Fellow, 2011
University of Illinois, Urbana-Champaign, Postdoctoral Fellow, 2015
University of Chicago, Assistant Professor, 2016-
RNA molecules play highly diverse functions in living cells, including carrying genetic information (mRNA), performing catalytic functions (e.g. ribozyme and ribosome) and regulating gene expression (e.g. various types of noncoding RNAs). Our research is centered on RNA biophysics and cell biology with cutting-edge single-molecule and cell imaging techniques.
Bacterial small RNA Small regulatory noncoding RNAs (here broadly defined as sRNAs) play critical roles in regulating genes involved in almost all cellular processes. Due to their specificity and versatility, sRNAs have inspired broad applications including new therapies for human diseases and genome engineering, among other applications. That said, a precise quantitative description of the fundamental mechanism of sRNA-mediated regulation and interference can largely benefit the further improvement of the efficiency and robustness of these applications by providing critical models and parameters. Our research aims at providing a quantitative description at the molecular, cellular, and the systems levels. Using bacteria as model systems, our missions are to understand the molecular mechanisms by which sRNAs modulate gene expression, as well as physiological response caused by sRNA-mediated regulation in the context of pathogenic bacteria-host interactions.
RNA/protein granule In eukaryotic cells, RNAs and proteins can self-assemble into various types of non-membranous structures or domains under natural or stressed conditions, including P body and stress granule, etc. in cytoplasm, and nucleolus, nuclear speckle, paraspeckle, and Cajal body, etc. in nucleus. As one of the most conspicuous domains in the nucleus, nuclear speckles are highly heterogeneous in composition, enriched with poly A+ RNAs and numerous proteins involved in mRNA processing. Many cellular functions have been implicated to be associated with nuclear speckles, including the storage and/or assembly sites of pre-mRNA processing factors, as well as the structural domains that control the efficiency and integration of distinct steps in gene expression, ranging from transcription to mRNA export. We aim at mapping the distribution of components within the speckle using cell imaging techniques, and determining the involvement of such organization in the speckle-ascribed functions. Furthermore, with such imaging-based quantitative analysis, we are interested in understanding the biophysical properties of RNA/protein granules in general.